Fig. 7.

Mutation of Tyr residue at 992 reduces basal and stimulated PTHrP reporter gene expression. A transient transfection assay comparing the reporter activity of the vector, wild-type EGFR (EGFR), and EGFRY992F mutant transfected cells with and without the addition of AREG (100 nM) or EGF (10 nM). The luciferase activities were normalized to β-galactosidase levels and were expressed as means ± s.d. of triplicate cultures. RLU, relative luciferase units. *P < 0.01 vs. EGFR unstimulated ^&P < 0.01 vs. EGFR stimulated with AREG or EGF