Figure 2. AC neurons are thermosensors.
a, AC (arrowhead), LC (arrow) and VC (double arrow) neurons. b, dTRPA1 minigene expression. c, AC locations (antennae removed). d, AC projections. e, Left, AC processes labeled using dTrpA1SH-Gal4;UAS-myr:RFP. Also labeled are cells that express dTrpA1SH-Gal4, but do not detectably express dTRPA1 protein [also see a]. Right, camera-lucida-style outline of AC projections. f–g, AC projections to AL (f) and SOG (g). h–j, G-CaMP-labeled AC’s. Two AC’s sometimes imaged simultaneously (h). k–l, Warmth-responsive G-CaMP fluorescence of AC’s. m, Maximum F/F each AC imaged. AL, antennal lobe; SOG, subesophageal ganglion; SLPR, superior lateral protocerebrum; eso (asterisk), esophagus.