Figure 3.

Reduced adipose tissue inflammation and hepatic steatosis in F-ACREB mice. A. Histological analysis of hematoxylin-eosin (H&E) stained WAT sections from HFD-fed F-ACREB (tg) and control (wt) littermates showing relative accumulation of inflammatory cell infiltrates. B. Top, insulin-stimulated glucose uptake in primary cultured adipocytes from F-ACREB transgenic (tg HFD) and control (wt HFD) littermates under HFD conditions. Relative uptake of ³H-2 deoxyglucose (4μCi/ml; expressed as cpm/10⁶ cells) into cultured adipocytes from HFD-fed mice exposed to various concentrations of insulin for 20 minutes followed by incubation with ³H-2 deoxyglucose for 5 minutes. ( *; P<0.002 relative to wild-type HFD fed mice; data are means ± s.e.m.) Bottom, immunoblot of GLUT4 protein amounts in adipose and quadriceps muscle from F-ACREB (Tg1, Tg2) and wild-type (WT1, WT2) littermates maintained under HFD conditions. C. Top, Q-PCR analysis of adiponectin mRNA amounts in adipose tissue from F-ACREB (tg HFD) and wild-type (wt HFD) littermates maintained under high fat diet conditions (n=3 per group; *, P<0.05). Bottom, circulating plasma adiponectin concentrations in fasted or 2 hour refed F-ACREB and control mice. D. Top, immunoblot showing phospho-AMPK amounts in liver lysates from wild-type (wt), ob/ob (wt-ob) and F-ACREB transgenic (tg-ob) mice. Amounts of unphospho- and phospho-CRTC2 also indicated. Bottom, hepatic sections from F-ACREB transgenic ob/ob and control ob/ob mice showing relative accumulation of lipid droplets. E. Acetoacetate content in livers of F-ACREB ob/ob and control ob/ob mice relative to lean wild-type animals (n=4). ( *; P<0.05 transgenic relative to control mice; n=4; data are means ± s.e.m.). F. Q-PCR analysis of beta oxidation (PGC1α, PPARα, UCP2) and gluconeogenic (PEPCK, G6Pase) gene expression in livers from ob/ob F-ACREB transgenic mice relative to ob/ob controls.