Figure 3.

Maintenance of transduced T-cell receptor (TCR) expression with repetitive in vitro stimulation. P14 T cells transduced with the TCR_αgag receptor chains were incubated with antibodies to CD8α and either the introduced, gag-specific TCR chains (Vα3 and Vβ12) or the endogenous gp33-specific TCR chains (Vα2 and Vβ8). (a) Expression of the gag-specific TCR on day 9 following the 8th in vitro stimulation when cells had been in culture for ~2.5 months. (b) Expression of the gp33-specific TCR at the same time point as in a. All plots shown are gated on CD8⁺ lymphocytes and the numbers in the histograms indicate the mean fluorescence intensity of α- and β−chain expression. F2A, foot and mouth disease virus–derived 2A peptide; IRES, internal ribosomal entry site.