Analysis of long-term persistence and function of transferred cells in treated mice. On day 214 following tumor challenge, splenocytes from surviving treated mice from Figure 4 were stimulated in vitro with irradiated FBL or gp33 peptide. Six days later, cells were restimulated with no peptide (φ), gag peptide, or gp33 peptide for 6 h and analyzed for production of IFN-γ and expression of Thy1.1. All transduced and adoptively transferred P14 T cells were derived from T cells expressing the Thy1.1 congenic marker. (a–d) Mice received Vα3IRESVβ12-transduced P14 T cells and (e,f) mice received Vα3F2AVβ12-transduced T cells. All plots are gated on CD8+ lymphocytes. IFN-γ, interferon-γ.