Table 1.
Ac-apoLp-III | unmodified apoLp-III | |
---|---|---|
molecular mass | 17,661 Da | 17,327 Da |
[Gdn-HCl]½ lipid-free | 0.65 M | 0.55 M |
[Gdn-HCl]½ DMPC-bound | 4.2 M | 4.4 M |
helical content | 54 % | 78 % |
helical content DMPC bound | 92 % | 99 % |
rate of DMPC transformation | 0.26 min−1 | 0.27 min−1 |
rate of DMPG transformation | 0.39 min−1 | 0.91 min−1 |
LDL protectiona | 25 μg | 10 μg |
The amount of apoLp-III required to prevent aggregation of 100 μg LDL treated with 2 mU of phospholipase-C during a 45 min incubation at 37 °C.