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. 2009 Aug 5;8:60. doi: 10.1186/1476-4598-8-60

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Copyright © 2009 Siam et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Overexpression of Lats1 in NMuMG epithelial cells does not hinder cell cycle progression. (A) RNA was prepared from NMuMG mouse epithelial cells expressing p110 CUX1 or carrying an empty vector. Lats1 mRNA expression was analyzed by RT-PCR using GAPDH as a control. (B) Cells were synchronized at the G1/S transition using the double thymidine block procedure, and then released from the block and allowed to progress in the cell cycle. Nuclei were stained with propidium iodide and DNA content was determined by fluorescence-activated cell sorting (FACS) analysis. Cell cycle profiles were analyzed using the Watson model and the FlowJo™ software. The numbers below the FACS profiles indicate the percentage of cells with 2N DNA content (G0/G1 cells), between 2N and 4N DNA content (S phase cells) and 4N DNA content (G2/M cells).