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. 2009 Sep;175(3):1019–1029. doi: 10.2353/ajpath.2009.080832

Figure 6.

Figure 6

BAPN and PBN affect Pyd crosslink formation, phosphorylation status of FAK, and actin filament formation in NHF and KSS dermal equivalents. NHF and KSS fibroblasts were incubated for 24 hours in the presence of 10 mmol/L BAPN. Then, cells were harvested and seeded into collagen gels with or without BAPN. PBN was added directly to the contraction medium at a concentration of 10 mmol/L. After 6 hours, skin equivalents were collected and prepared for immunohistologic analysis of Pyd crosslinks (A), phospho-FAK [Y397] (A), and actin filaments using phalloidinFITC (B). Controls with secondary antibody confirmed specificity of Pyd and phospho-FAK staining. Scale bar = 20 μm.