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A: Liver sections of control and BDL mice were analyzed for CD68 expression and CD11b expression (Original magnification, ×200). Infiltrating neutrophils were also detected by (arrows) Naphthol ASD staining. B: Quantitative RT-PCR was performed by using pooled RNA extracted from wild-type (WT) and Bid−/− mice before (open bars) and after BDL (closed bars) (n = 4). Specific cDNAs of KC, monocyte chemotactic protein-1, interleukin-6, and TNF-α were amplified.
