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. 2009 Sep;175(3):1246–1254. doi: 10.2353/ajpath.2009.090042

Figure 5.

Figure 5

Targeted overexpression of EZH2 impairs mammary gland involution. A: H&E-stained sections of the mammary glands of wild-type and MMTV-EZH2 mice at the indicated day of involution (i) (n = 5 mice/group/day were analyzed). Note the persistence of amorphous debris and secretory lobules in the mammary glands of MMTV-EZH2 mice at all phases of involution. B, a and b: Whole-mount carmine-stained mammary glands from EZH2 overexpressing mice at day 14 of involution retained epithelial clusters and enlarged ducts filled with membranous materials (arrows). c and d: H&E-stained sections of MMTV-EZH2 and wild-type mammary glands show that although wild-type glands underwent complete involution at day 14, MMTV-EZH2 retained abnormally dilated ducts with eosinophilic proteinaceous material. e and f: CD68 staining by immunohistochemistry reveals that MMTV-EZH2 mammary glands at day 14 of involution have multiple macrophages indicative of persistent involution, whereas there are no remaining macrophages in the wild-type gland.