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. 2009 Sep;175(3):1281–1291. doi: 10.2353/ajpath.2009.081110

Figure 6.

Figure 6

Gelatinases and collagenases in the serum-free conditioned media of organotypic cultures. The collagen and myoma organotypics were cultured in the absence (−) or presence (+) of carcinoma cells (HSC-3), and the media were collected every 3 days (days 4 and 14 shown) and concentrated. A: Gelatin zymography for MMP-2 and MMP-9. Purified gelatinase standards are shown on the right: pro-MMP-2 (72 kDa), active MMP-2 (62 kDa), pro-MMP-9 (92 kDa), and active MMP-9 (82 kDa). Western blotting for MMP-1 (latent 52 kDa and active 41 kDa) (B), (C) for MMP-13 (latent 60 kDa), and (D) MMP-8 (latent 54 kDa). Protein molecular weight standards (55 kDa) are shown on the right in B–D.