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. 2009 Aug 31;4(8):e6846. doi: 10.1371/journal.pone.0006846

Figure 4. IL-10 induced STAT3 activity is insensitive to JSI-124 inhibition.

Figure 4

JSI-124 treated and untreated monocytes (as described in figure 3) were cultured with or without IL-10 (10 ng/ml). After 2 hours, cells were harvested and either lysed and their nuclear extracts were tested for STAT3 activity using Non-Radioactive STAT3 Transcription Factor Assay (left panel) or phosphorylated STAT3 was detected by intracellular immunostaining and flow cytometric analysis (right panel). Dashed line: Isotype control; Filled grey line: monocytes alone; Thick grey line: IL-10 stimulated monocytes; Black line: IL-10 stimulated JSI-124 pretreated monocytes. Comparable results were obtained in three independent experiments.