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. 2009 Sep 3;4(9):e6885. doi: 10.1371/journal.pone.0006885

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Sørby et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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PrP mRNA expression levels in compartments of spleen of five VRQ/VRQ lambs after oral inoculation with scrapie (black columns) and five control lambs (gray columns). The y axis represents mRNA levels relative to GAPDH expression obtained by real-time RT-PCR analysis. Error bars represent standard deviation. There was no significant difference in PrP mRNA levels between the scrapie-inoculated and control animals. The level of PrP mRNA expression in the red pulp (RP) was significantly higher than in all other tissue compartments, namely the periarterial lymphatic sheaths (PALS), lymphoid nodules (LN), marginal zone (MZ) and trabeculae (TR).