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. Author manuscript; available in PMC: 2009 Aug 25.
Published in final edited form as: J Biol Chem. 2002 Jun 5;277(34):31207–31213. doi: 10.1074/jbc.M204114200

Fig. 2. Expression, purification, and immunoreactivity of rSmTCTP.

Fig. 2

A, ~20 μg of protein extracted from uninduced (Uind) and induced (Ind) cultures of Escherichia coli containing SmTCTP expression construct were separated in a 12% SDS-PAGE. rSmTCTP was then purified from the cultures using a nickel-nitrilotriacetic acid chromatography column, and ~1 μg of the protein was separated in a 12% SDS-PAGE (rSmTCTP). B, affinity-purified rSmTCTP probed with sera collected from C57BL/6 mice on day 0 or week 2, 3, 8, or 9 after infection with S. mansoni. Purified rSmTCTP probed with a mouse anti-SmTCTP serum served as a positive control.