Table 2.
Summary of Unique Glycated Peptides Considered as Up-Regulated in IGT and T2DM Groups from Erythrocyte Membrane Samplesa
| gene | accession no. | protein description | peptide sequence | glycation site |
R. T. (min) |
S. C. NGT |
S. C. IGT |
S. C. T2DM |
|---|---|---|---|---|---|---|---|---|
| ANK1 | IPI00216697.2 | ankyrin 1 isoform 1 | (R)AGHTEVAkYLLQNK(A) | K454 | 26.7 | 2 | 5 | 4 |
| ANK1 | IPI00216697.2 | ankyrin 1 isoform 1 | (R)VAkVLLDK(G) | K355 | 16.9 | 0 | 2 | 2 |
| EPB41 | IPI00003921.2 | isoform 1 of protein 4.1 | (K)HAkGQDLLK(R) | K231 | 8.8 | 0 | 0 | 2 |
| GAPDH | IPI00219018.6 | glyceraldehyde-3-phosphate dehydrogenase | (K)AGAHLQGGAkR(V) | K116 | 2.9 | 1 | 2 | 2 |
| GAPDH | IPI00219018.6 | glyceraldehyde-3-phosphate dehydrogenase | (K)AVGkVIPELNGK(L) | K218 | 26.4 | 1 | 2 | 2 |
| GAPDH | IPI00219018.6 | glyceraldehyde-3-phosphate dehydrogenase | (K)FHGTVkAENGK(L) | K60 | 5.3 | 2 | 6 | 5 |
| GAPDH | IPI00219018.6 | glyceraldehyde-3-phosphate dehydrogenase | (K)VkVGVNGFGR(I) | K4 | 23.3 | 0 | 1 | 1 |
| HBD | IPI00473011.2 | hemoglobin subunit delta | (K)VVAGVANALAHkYH(−) | K144 | 27.5 | 0 | 0 | 4 |
| MPP1 | IPI00215610.2 | 55 kDa erythrocyte membrane protein | (R)SHIkNALLSQNPEK(F) | K299 | 20.1 | 0 | 2 | 1 |
| SPTA1 | IPI00220741.4 | spectrin alpha chain, erythrocyte | (K)ALkAQLIDER(T) | K1486 | 23.5 | 1 | 2 | 3 |
| SPTB | IPI00216704.6 | isoform 2 of spectrin beta chain, erythrocyte | (K)EkVQLIEDR(H) | K1247 | 19.9 | 1 | 2 | 2 |
| SPTB | IPI00216704.6 | isoform 2 of spectrin beta chain, erythrocyte | (K)NNEkAQEASVLLR(D) | K1261 | 22.9 | 1 | 2 | 2 |
| SPTB | IPI00216704.6 | isoform 2 of spectrin beta chain, erythrocyte | (R)LQGQVDKHYAGLkDVAEER(K) | K1675 | 24.3 | 0 | 1 | 2 |
| SPTB | IPI00216704.6 | isoform 2 of spectrin beta chain, erythrocyte | (R)TQLVDTADkFR(F) | K1904 | 25.4 | 1 | 2 | 1 |
a
Modified amino acids are shown in lower case; k, Amadori modified lysine; m, oxidized methionine. R. T. stands for LC retention time; S. C. stands for scan counts in MS/MS analyses.