Table 6. Inhibition of MBNL1-RNA11 (DM1) and RNA12(SCA3) Interactions with 5H-4. The IC50's are reported in μM.
Ligand | Condition Aa | Condition Ba | ||
---|---|---|---|---|
RNA 11 | RNA 12 | RNA 11 | RNA 12 | |
2 | 30.2 ± 6.8 | 48.4 ± 9.9 | 38.1 ± 6.9 | 21.1 ± 0.5 |
5H-4 | 0.25 ± 0.01 | 0.16 ± 0.03 | 0.13 ± 0.01 | 0.13 ± 0.06 |
Multivalent Enhancements b | ||||
5H-4 | 24 | 60.5 | 58 | 32 |
In condition A, 7.5 pmoles of MBNL1 are added to each well; in condition B, 2.0 pmoles of MBNL1 were added per well.
Calculated as described in the Experimental.