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. 2009 Jun 5;58(9):1953–1960. doi: 10.2337/db08-1293

FIG. 1.

FIG. 1.

Incubation with IL-6 increases α2 AMPK activity, protein phosphorylation, and UCP3 and PGC1α protein abundance in incubated EDL muscle. A: α2 AMPK activity (bar graph) and AMP:ATP ratio (line graph) were assayed in rat EDL muscles incubated with IL-6 (120 ng/ml) for 15–60 min. B: Dose-curve of α2 AMPK activity in response to IL-6 (15–120 ng/ml) for 30 min. C: Immunoblots of P-ACC (Ser79), P-STAT3 (Tyr705), P-AMPK (Thr172), P-CREB (Ser133), and β-actin in EDL muscles incubated with IL-6 (120 ng/ml) for 15 min. D: Immunoblots of PGC1α, UCP3, and β-actin in EDL incubated with IL-6 (120 ng/ml) for 30–120 min. E: Densitometric analysis of immunoblots of UCP3 and PGC1α protein shown in D. Results are means ± SE, n = 4–10. EDL muscles. *P < 0.05 versus control. ‡P < 0.05 versus 15 min IL-6. Immunoblots shown are representative of four samples.