FIG. 1.

Expression of ZnT8 in human pancreatic slices (A) and dissociated islet cells (B) and in purified wild-type mouse β- and α-cells (C). A: Pancreatic slices stained for ZnT8; scale bar, 50 μm. Cells in which clear colocalization to non–β-cells was apparent are highlighted with arrows. Essentially identical data were obtained with isolated human islets (not shown). B: Human islets were isolated (26) and dissociated with trypsin to allow the staining of single cells; scale bar, 5 μm. C and D: Mouse pancreatic α- and β-cells collected by flow cytometry from transgenic mice expressing the variant yellow fluorescent protein Venus under the control of the preproglucagon promoter (see supplementary Methods). Three separate preparations of α- and β-cells were analyzed by either microarray analysis (C) or qPCR (D). D: Expression is presented relative to that of β-actin measured in the same sample. Primer and probe sequences are available on request. *P < 0.05 β- versus α-cell normalized mRNA levels. NS, not significant. (A high-quality digital representation of this figure is available in the online issue.)