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. 2009 Jul 6;58(9):2006–2017. doi: 10.2337/db09-0323

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© 2009 by the American Diabetes Association.

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FIG. 1. — C3aR mRNA expression is modulated by an HFD. Total RNA was extracted from various tissues from lean (n = 10) and obese (n = 10, 8–16 weeks on HFD) mice. A: Total eWAT, adipocyte-purified fraction, and the SVC fraction. B: Quantitative PCR on aP2/FABP4 (adipocyte marker) and F4/80 (macrophage marker) confirmed the proper isolation of each fraction. C: BAT. D: Muscle. E: Liver. F: Brain. mRNA expression was examined by quantitative RT-PCR (Taqman) and normalized to cyclophilin B expression. Each PCR was performed in duplicate. The following probes from Applied Biosystems were used: cyclophilin B, Mm00478295_m1; F4/80, Mm00802530_m1; CD68, Mm00839636_g1; and C3aR, Mm02620006_s1 and Mm01184110_m1. *P < 0.05; **P < 0.01; ***P < 0.001 by unpaired t test.