FIG. 4.

ITT and OGTT in C3aR^−/− mice. Nine-week-old wild-type (WT) and C3aR^−/− males (n = 6 per group) for ITT on ND (A) and on an 8-week HFD (B) were starved for 4 h and injected with one dose of insulin (0.3 units/kg i.p.). A: ●, wild type; △, C3aR1^−/− ND. B: ●, wild type HFD; △, C3aR^−/− HFD. For OGTT, mice were starved for 24 h and then administered one dose of glucose (2 g/kg p.o.) on 2 weeks HFD (C) and on 8 weeks HFD (D). C: ●, wild type; △, C3aR^−/−. D: ●, wild type HFD; △, C3aR^−/− HFD. Blood glucose was measured from tail vein after 0, 20, 40, 60, and 120 min. AUC was calculated. *P < 0.05; **P < 0.01; ***P < 0.001 by ANOVA.