FIG. 2.

Effect of luminal RELM-β on glucose-induced Isc. Rat jejunal mucosa was mounted in Ussing chamber and the increase in Isc was studied at steady state. Electrogenic (Na⁺) transport was followed as an index of the active glucose transport by cotransporter SGLT-1. RELM-β was added in the mucosal bath 2 min before challenging the tissues with 10 mmol/l glucose. Values for Isc were standardized to control values and expressed as percentage of controls. A: Dose-dependent inhibition. B: The effect of 0.1 nmol/l RELM-β after an overnight incubation with an antibody against RELM-β. C: Representative SGLT-1 immunoblot of solubilized BBMs. BBMs were prepared from rat jejunum loops incubated in situ with and without luminal RELM-β during 6 min. Densitometric analysis of immunoreactive bands was performed using National Institutes of Health Image analysis program. The densitometry represents the amount of SGLT-1 relative to β-actin and is representative of at least three separate experiments. Each point of the Isc study represents the means ± SE of four to eight noncumulative values from five separate experiments. Significant differences from control, *P < 0.05, **P < 0.01.