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. 2009 Sep 3;4(9):e6859. doi: 10.1371/journal.pone.0006859

Figure 6. Induction of IL-8 transcription in AGS cells.

Figure 6

AGS cells were infected with wild type or isogenic mutant H. pylori strains or were mock infected (Control), and levels of IL-8 mRNA, normalized relative to β-2 microglobulin mRNA, at the indicated times were determined by real time PCR with primers listed in Table S1D. These analyses showed that AGS cell infection by strain PeCan18B caused strong rapid IL-8 induction, and that this induction depended on the virB10 gene of this strain's cag PAI, but not on tfs3 nor TnPZ; in contrast, infection by HUP-B43, which naturally lacks a cag PAI caused a gradual, TnPZ-dependent IL-8 induction.