. Author manuscript; available in PMC: 2009 Aug 25.

Published in final edited form as: Nat Cell Biol. 2006 Jul 23;8(8):793–802. doi: 10.1038/ncbl439

Table 1.

Identification of genes involved in RNAi function in Drosophila S2 cells

			Readout^b
Group^a	Gene ID	Gene name	Relish–GFP	LAMP GFP	dlAP1	Luciferase
Proton transport	CG3161	Vhal6	+++	+++	++	+++
	CG17332	VhaSFD	++	+	+	++

Vesicle mediated transport	CG9012	Clathrin he	+++	+++	++	+++
	CG7057	AP-50	++	++	++	++
	CG5915	Rab7	++	++	++	++
	CG6025	Arf72A	+	+	++	+++

Intracellular transport	CG54125	ninaC	+	+	++	++
	CG6177	IdlCp	+++	++	+	+
	CG3248		+++	++	++	++
	CG3911		+	++	+	++
	CG18028	light	+	+	+	+

Lipid metabolism	CG3495	Gmer	+	+	++	+++
	CG5373	Pi3K59F	+	+	++	+++
	CG12070	Saposin-r	++	+++	+++	+++

Proteolysis and peptidolysis	CG4572		+++	+++	+++	+++
	CG5053		+	+	++	++
	CG8184		+	+	++	++
	CG8773		+	+	++	++

Other	CG9659	egghead	+++	+	++	++

Unknown	CG5161		++	+	++	+
	CG5382		++	+	++	++
	CG5434		+++	+++	++	++
	CG8671		+	+	++	++

RNAi function	CG6493	Dicer 2	+++	++	+	++
	CG4792	Dicer 1	+	++	++	++
	CG7439	Argonaute 2	+++	+++	+++	+++

Control			−	−	−	−

Genes were classified according to their biological function as annotated in Flybase (http://flybase.bio.indiana.edu/annot/).

Inhibition of RNAi function was assayed using four different secondary RNAi assays. Score: +++, expression of reporter gene equal to control in which expression of marker genes is not suppressed by RNAi; ++ and +, intermediate levels of expression of the reporter gene; −, expression of the reporter gene was silenced to the same level as control that only received marker gene RNAi treatment.