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. Author manuscript; available in PMC: 2010 Jun 16.
Published in final edited form as: Biochemistry. 2009 Jun 16;48(23):5396–5404. doi: 10.1021/bi900253d

Figure 6. AP endonuclease 2 may serve a redundant function for AP endonuclease 1 during very early development.

Figure 6

qRTPCR was performed to examine expression levels of apex 1, apex 2 and gapdh in early stage, untreated embryos (3.5 hpf). ΔCT values were calculated by normalizing to gapdh for the embryo and adult fish. Relative gene expression (plotted), ΔΔCT, was calculated by subtracting the ΔCT from the the ΔCT of the embryo for apex1, apex 2, and gapdh. Data are means of triplicate values calculated from at least one primer pair of each gene over two independent experimental runs. Error bars indicate standard error of the mean.