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. Author manuscript; available in PMC: 2010 Jul 17.
Published in final edited form as: Immunity. 2009 Jul 17;31(1):158–169. doi: 10.1016/j.immuni.2009.04.016

Figure 3. CD4+ T cells primed in the presence of IL-12 help B cells.

Figure 3

(A) Naive B cells pre-activated with anti-IgM were cultured in the presence of CpG and SEB with autologous CD4+ T cells primed with the indicated cytokines. Ig titers in the supernatants were measured at day 14. Mean ± s.e.m. n=6. Unpaired two-tailed t-test. A representative out of three experiments.

(B) Memory B cells were cultured in the presence of SEB with autologous CD4+ T cells primed with the indicated cytokines. Ig levels at day 14. Mean ± s.e.m. n=6. Unpaired two-tailed t-test. A representative out of three experiments.

(C) Ig secretion from memory B cells cultured with CD4+ T cells primed with titrated amounts of IL-12. A representative out of two experiments.

(D) IL-21R-Fc was added to block IL-21 to the co-cultures of memory B cells and CD4+ T cells primed with IL-12. Ig levels at day 14. Mean ± s.e.m. n=6. Unpaired two-tailed t-test. A representative out of three experiments.

(E) Blocking of ICOS by ICOS-L-mIgFc during T-B cultures. Ig levels at day 14. Mean ± s.e.m. n=6. Unpaired two-tailed t-test. A representative out of three experiments.