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Automatic trabecular bone ROI segmentation procedure illustrated for the distal tibia. (a) Manually selected rectangular region centered on the ROI; (b) Overlapping grid pattern for applying local threshold; (c) Initial local threshold mask, roughly identifying cortical shell; (d) Dilation of mask in (c) to avoid leakage; (e) Selection of main connected component; (f) Final ROI after filling small holes.
