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. Author manuscript; available in PMC: 2009 Aug 25.
Published in final edited form as: Neuroendocrinology. 2008 Oct 8;89(2):210–216. doi: 10.1159/000162916

Figure 4. Endopin 2 colocalization with neuropeptides in secretory vesicles of chromaffin cells demonstrated by immunofluorescence confocal microscopy.

Figure 4

a. Endopin 2 and enkephalin. Immunofluorescence colocalization of endopin 2 (E2) with the enkephalin neuropeptide in chromaffin cells (bovine) was assessed by rabbit anti-endopin 2 detected with anti-rabbit IgG-Alexa Fluor 488 (green fluorescence), and detection of (Met)enkephalin (Enk) with mouse anti-Enk detected with anti-mouse IgG Alexa 594 (red fluorescence). The colocalization of endopin 2 and (Met)enkephalin is illustrated by overlay of the images, illustrated by yellow fluorescence.

b. Endopin 2 and NPY. Immunofluorescence colocalization of endopin 2 (E2) with the NPY neuropeptide in chromaffin cells (bovine) was assessed by rabbit anti-endopin 2 detected with anti-rabbit IgG Alexa Fluor 488 (green fluorescence), and detection of NPY with sheep anti-NPY detected with anti-sheep IgG Alexa 594 (red fluorescence). The colocalization of endopin 2 and NPY is illustrated by overlay of the images, illustrated by yellow fluorescence.

c. Endopin 2 and chromogranin A. Immunofluorescence colocalization of endopin 2 (E2) with the secretory vesicle marker chromogranin A (CgA) in chromaffin cells was assessed by rabbit anti-endopin 2 detected with anti-rabbit IgG Alexa Fluor 488 (green fluorescence), and detection of CgA with mouse anti-CgA detected with anti-mouse IgG Alexa 594 (red fluorescence). The colocalization of endopin 2 and CgA is illustrated by the overlay of the images, illustrated by yellow fluorescence.