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hSERT forms a stable association with PKGIα. hSERT and HA-PKGIα or HA-PKGIβ were coexpressed in HEK-293 T cells and coimmunoprecipitations were performed as described in Methods. Total cell extracts were blotted for A) HA to reveal PKG expression or B) SERT. C) Co-immunoprecipations of SERT and PKG using anti-HA for immunoprecipitations and anti-SERT for blotting. Experiment shown was replicated in four independent experiments with similar results.
