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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Leuk Res. 2008 Dec 2;33(7):991–996. doi: 10.1016/j.leukres.2008.10.022

Figure 3. Insertional sequences stimulate reporter gene expression.

Figure 3

HEK293T cells were transiently transfected with luciferase reporter plasmid having the HSV TK promoter with the first intron from +34 to +150 (2R) of the ASNS gene either with or without additional 3R and 4R insertional sequences. Firefly luciferase expression levels were normalized to the level of Renilla luciferase expression. Assays were done in triplicate and repeated in three independent experiments. Abbreviations: 2R; pGL3-TK-2R plasmid, 3R; pGL3-TK-3R plasmid, 4R; pGL3-TK-4R plasmid, and *; p < 0.01.