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Confocal laser scanning microscopy of HeLa cells (A–C), transfected with pEGFP-N1. In A, cSCK was used as the transfection agent with pEGFP-N1 at an N/P ratio of 6:1. B and C were positive controls using Polyfect and Lipofectamine 2000 as the transfection agent, respectively, at N/P ratios recommended by the manufacturers.
