Figure 4. Target deletion of palladin does not inhibit neurite extension from primary cortical neurons in culture.

Fluorescence immunostaining of Tuj1 in wild-type (wt, A) and palladin-deficient (ko, B) primary cortical neurons (4DIV). Representative examples of GFP transfected wt (C) and ko (D) neurons (4DIV) are shown. Quantification of neurite outgrowth based upon the GFP positive neurons described above. Cells with neurites of longer than two soma diameters were scored as long neurite-bearing cells. The percentage of long neurite-bearing cells is not decreased in palladin-deficient neurons (E). The length of the longest neurite is not significantly reduced in palladin-deficient neurons (F). Results are expressed as the mean±SEM (n = 3).