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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2009 Jul 7;8(7):2067–2075. doi: 10.1158/1535-7163.MCT-08-1217

Figure 4.

Figure 4

Rapid response of p53 in SAM486A-treated NB cells. Whole cell lysates were obtained from SK-N-SH cells exposed to (A) 10 μM SAM486A for 2, 4, 8, 16, and 24 hours or (B) exposed to increasing concentrations of SAM486A (0, 0.1, 1, 2, 5, and 10 μM) for 72 hours, and analyzed by Western blot. Resolved proteins were transferred to PVDF membranes and sequentially probed for total p53, phospho-p53 (Ser392), and phospho-p53 (Ser46). SAM486A treatment increased total p53, phospho-p53 (Ser392) and phospho-p53 (Ser46) in a time-and dose-dependent manner. These effects were observed as early as 8 hours after treatment with 10 μM SAM486A, and at concentrations as low as 1-2 μM SAM486A. GAPDH or β-actin served as loading markers. C, the bands were quantified using a Bio-Rad Multi Imager and Quantity One Quantitation Software (Bio-Rad), and normalized to the untreated control group. Western blot data are representative of three independent experiments (n=3).