Figure 5.

Effects of the individual adipogenesis-inducing reagents upon TMD expression changes during adipogenesis. Relative expression changes of adipogenesis-related TMDs and two master transcription factors (PPARG2 and CEBPA) after 24 h treatment with none, each, or all of the four adipogenesis-inducing reagents (dexamethasone, Dex; insulin, Ins; indomethacin, Ind; isobutylmethylxanthine, IBMX) were quantified by real-time RT–PCR. For the TMDs and CEBPA, expression levels induced by any of the reagents are indicated relative to those in hMSCs that were treated with none of the adipogenesis-inducing reagents (None). Because PPARG2 showed a detectable expression level only with Dex and all four reagents, the former expression level (Dex) is indicated relative to the latter one (All), which is normalized to 100. ND, not detected. See Table S1 for primer sequences. Error bars show standard deviations.