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. 2009 Jun 16;37(15):4987–5000. doi: 10.1093/nar/gkp426

Figure 6.

Figure 6.

Dissection of upstream-signaling pathways regulating TMD transcription. (A) TMD expression changes by Wnt activation. Expression levels of all the adipogenesis-related TMDs and Wnt target genes (PPARG2 and CEBPA) were measured using real-time RT–PCR 24 h after adipogenic induction in the absence or presence of Wnt3A (150 ng/ml). The log ratio of the individual TMD expression with versus without Wnt3A is shown on the y-axis. (B) TMD expression changes by PKA activation. Expression levels of all the adipogenesis-related TMDs and a PKA target gene (PTGS2) were measured using real-time RT–PCR after 7-day treatment of hMSCs with forskolin (50 μM) or vehicle control (dimethyl sulfoxide). The ratio of the individual TMD expression with versus without forskolin is shown on the y-axis. See Table S1 for primer sequences. Error bars show standard deviations.