Table 2.
Fidelities observed for DNA polymerases using pSJ1(+/–)
| Polymerase/plasmid combination | Total coloniesa | Mutant (white) coloniesa | Observed mutation rate (%)b | Corrected mutation rate (%)c |
|---|---|---|---|---|
| Taq-Pol/pSJ1(+) | 4418 | 43 | 1.0 ± 0.14 | 0.9 |
| T4-Pol/pSJ1(+) | 3855 | 16 | 0.4 ± 0.14 | 0.3 |
| Pfu-Pold/pSJ1(+) | 4824 | 10 | 0.2 ± 0.02 | 0.1 |
| Taq-Pol/pSJ1(–) | 4173 | 43 | 1.0 ± 0.3 | 0.9 |
| T4-Pol/pSJ1(–) | 4567 | 18 | 0.4 ± 0.14 | 0.3 |
| Pfu-Pold/pSJ1(–) | 4549 | 10 | 0.2 ± 0.02 | 0.1 |
| Pfu-Pol (exo–)/pSJ1(+) | 3836 | 52 | 1.3 ± 0.09 | 1.2 |
| Pfu-Pol (exo–, Q472G)/pSJ1(+) | 3485 | 58 | 1.7 ± 0.2 | 1.6 |
| Pfu-Pol (exo–, D473G)/PSJ1(+) | 3527 | 109 | 3.1 ± 0.15 | 3.0 |
aThe numbers of total and white colonies are summed from three independent observations in each case.
bObserved mutation rate is defined as (white colonies)/(total colonies) × 100. The figures given are the averages for the three observations ± SD.
cThe corrected mutation rate has had 0.08% (the average background mutation rate, Table 1) subtracted from the observed value.
dThe Pfu-Pol used in these experiments was Phusion, a derivative of the polymerase fused with a DNA-binding domain and obtained from New England Biolabs.