Table 3.
Mutation spectra observed using pSJ1(+/–)
| Polymerase/plasmid combination | Template mutation (number)a | Mismatch formed (template:dNMP) |
|---|---|---|
| pSJ1(+) gapped control | C→T (5) | C:A |
| Taq-Pol/pSJ1(+) | ΔG (2) | – |
| G→A (2) | G:T | |
| C→T (2) | C:A | |
| T4-Pol/pSJ1(+) | A→T (5) | A:A |
| Pfu-Pol/pSJ1(+) | A→T (2) | A:A |
| C→T (1) | C:A | |
| G→A (1) | G:T | |
| C→G (1) | C:C | |
| pSJ1(–) gapped control | C→T (5) | C:A |
| Taq-Pol/pSJ1(–) | C→T (5) | C:A |
| T4-Pol/pSJ1(–) | C→T (6) | C:A |
| Pfu-Pol/pSJ1(–) | C→T (2) | C:A |
| G→T (1) | G:A | |
| G→A (3) | G:T |
aFor each polymerase/plasmid combination, five separate mutant (white) clones had their entire lacZα gene (coding strand) sequenced. The changes observed together with their frequencies (number in brackets) are given. In most cases only one mistake was observed per lacZα gene. In two cases [T4-Pol/pSJ1(–) and Pfu-Pol/pSJ1(–)] one of the mutant genes contained two errors, accounting for the total of six mistakes.