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Effect of Spo0A on the in vitro transcription of the main early and late promoters of Nf. Promoter A2c (A), C2 (B), A2b (C), A3 (D). Reaction mixtures contained 4 nM of the appropriate template DNA containing the indicated promoter, 40 nM of purified B. subtilis RNAP and increasing amounts of Spo0A. Spo0A concentrations, in 4-fold dilution steps, ranged from 23 nM to 23.6 μM. In the case of the late A3 promoter, the reaction mixture also contained 2.3 μM of protein p4.
