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. 2009 Jun 26;37(15):5167–5182. doi: 10.1093/nar/gkp519

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Directed hydroxyl radical cleavage of 18S rRNA in 40S/eIF1A binary and 43S complexes from Fe(II) tethered to cysteines in the NTT, CTT and C-terminal subdomain of eIF1A. Primer extension analysis of directed hydroxyl radical cleavage of 18S rRNA in 40S/eIF1A binary complexes (A, C, E, G, I, K) and 43S complexes (B, D, F, H, J, L) assembled from 40S subunits, Met-tRNA_i^Met, eIF2, eIF3, eIF1 and Fe(II)-tethered eIF1A mutants. Positions, from which hydroxyl radicals cleaved 18S rRNA, are shown in bold. Positions of cleaved nucleotides are shown on the right. Lanes G, A, T, C depict 18S rRNA sequence generated from the same primer.