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Growth of UM341 (E. coli orn–) carrying a vector control or expressing RNase J1 in the presence of varying concentrations of IPTG
| Plasmid | +Atc IPTG in μM |
−Atc IPTG in μM |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 10 | 50 | 100 | 200 | 0 | 10 | 50 | 100 | 200 | |
| pBAD18 (vector) | ++++ | ++++ | ++++ | ++++ | ++++ | + | + | + | + | + |
| pDG148-rnjA (RNase J1) | ++++ | ++++ | +++ | ++ | – | ++ | ++ | – | – | – |
| pUM408 (Orn) | ++++a | ++++a | ||||||||
Colony sizes: ++++, wild type; +++, smaller; ++, very small; +, tiny; –, no growth, Atc: anhydrotetracycline.
aIn the presence of 0.02% arabinose.
