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. 2009 Jun 24;37(15):5126–5137. doi: 10.1093/nar/gkp530

Figure 2.

Figure 2.

Preferential inhibition of Topo IV leads to the accumulation of DNA catenanes. Autoradiograms of 2D gels corresponding to pBR18 isolated from DH5αF′ E. coli cells untreated (A) and after exposure to norfloxacin (B). A diagrammatic interpretation of the different signals in the autoradiograms is shown to the right (C) where CatAs are depicted in blue, CatBs in red and CatCs in green. RIs = nicked replication intermediates; OCd = open circles corresponding to dimers; OCm = open circles corresponding to monomers; Knm = nicked knotted monomers; Ld = linearized dimers; Lm = linearized monomers. Monomeric topoisomers are depicted in black.