Figure 2.

The Isoetes engelmannii mtDNA with protein-coding genes shown in blue, tRNAs in yellow, rRNAs in orange, pseudogenes in the respective lighter colours and the cp and nuc DNA inserts indicated with green and grey boxes, respectively. Drawing is approximately to scale. Genes shown above or below the lines indicate directions of transcription to the right or to the left, respectively. Recombination points (R1–R24) are highlighted in magenta with arrows indicating recombination forks. Selected connections between islands of recombination are exemplarily shown with stippled grey lines. Unique net mtDNA sequences add up to 57 571 bp. Arrowheads indicate oligonucleotide primers (1–3 and a–i) anchoring in regions not affected by RNA editing to analyze the arrangement of cox1 and its transcript maturation.