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. 2009 Sep 4;4(9):e6902. doi: 10.1371/journal.pone.0006902

Figure 4. Genotyping PCR using flanking primer sets.

Figure 4

(A) The position of primers. Dotted line shows genomic deletion (see results and discussion). (B) PCR with the primers N008/N009 identifies either transgenic or non-transgenic mice, in which the product is detected as 400 bp band. On the other hand, multiple PCR with three primer sets clearly distinguish wild-type (single larger band), homozygous (single smaller band) and hemizygous (both bands) DNA. The expected size of PCR products are 500 bp and 295 bp with Chr18F1/Chr18R2/OUT1, and 1.5 kb and 1 kb with Chr18F5/R2/R3.