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. Author manuscript; available in PMC: 2009 Aug 26.
Published in final edited form as: Int J Pept Res Ther. 2008 Dec;14(4):293–300. doi: 10.1007/s10989-008-9150-3

Table 1.

HPLC & MS data of Ac-MSH(7)-NH2, Ac-CCK(6)-NH2 and heterobivalent ligands 1–7

Compound Mass calculated Mass found tR (purity %) K′
Ac-MSH(7)-NH2 1015.5035 (M + 1)1+ 1015.4881a (M + 1)1+ 17.0d (96) 9.2
Ac-CCK(6)-NH2 791.4014 (M + 1)1+ 791.1a (M + 1)1+ 15.4e 7.7
1 (C107H145N27O25) 1105.0452 (M + 2)2+ 1105.2a (M + 2)2+ 20.3f (99) 11.9
2 (C128H175N33O31) 1336.1566 (M + 2)2+ 1336.3a (M + 2)2+ 19.2f (100) 11.3
3 (C149H205N39O37) 1045.1786 (M + 3)3+ 1045.1891b (M + 3)3+ 18.3f (98) 10.8
4 (C170H235N45O43) 1199.2529 (M + 3)3+ 1199.2559b (M + 3)3+ 18.0f (99) 10.6
5 (C191H265N51O49) 4059.9942* (M + 1)1+ 4060.5121c (M + 1)1+ 17.7f (87) 10.4
6 (C100H141N23O25) 1033.0235 (M + 2)2+ 1032.9a (M + 2)2+ 21.5f (100) 12.7
7 (C114H167N25O31) 1192.1129 (M + 2)2+ 1192.0a (M + 2)2+ 21.2f (100) 12.5

HPLC eluents: phase A (0.1% TFA in water); phase B (Acetonitrile); tR is the retention time of compound peak in HPLC; (purity of final product in percentage is given in parenthesis); K′ is retention time of compound peak/retention time of solvent peak

a

ESI-MS;

b

FT-ICR;

c

MALDI-MS;

d

10–40% B gradient in 30 min;

e

10–90% B gradient in 30 min;

f

20–60% B gradient in 50 min;

g

10–40% B gradient in 50 min

*

Mass of highest abundant isotope peak