A longitudinal section through the connecting piece. This and all subsequent specimens are from sections cut parallel to the freezing plane. This example resembles the pattern seen after conventional fixation except that the contrast is reversed – for unknown reasons. Thus, the normally dense and wide bands of the striated columns appear pale (large arrow) and the normally pale and thin bands appear dense (small arrow). (For continuity, the bands are nevertheless referred to as ‘dense’ and ‘pale’, respectively.) The periodicities in this specimen were rather uniform. The mean repeat periods, as measured, were 60.6nm (left side) and 59.0nm (right side). Scale bar=0.2µm.