Table 4.

Quantitative PCR analysis of viral gene expression

Gene	2hr	4 hr	9 hr	TS 5	CHX
18 K	223 ± 15.6	1248 ± 25.2	7646 ± 10.9	3695 ± 8.7	988 ± 6.5
ICP-46	253 ± 16.4	491 ± 9.2	14468 ± 5.1	28726 ± 7.6	7231 ± 8.5
vCARD	325 ± 5.1	7652 ± 11.3	65535 ± 5.5	99334 ± 5.5	6422 ± 5.0
vPol IIα	23 ± 2.0	74 ± 1.0	58 ± 3.2	337 ± 8.0	52 ± 4.9
vPol IIβ	37 ± 2.0	92 ± 4.1	156 ± 4.0	566 ± 6.6	81 ± 6.0
MCP	42 ± 2.5	154 ± 4.7	14274 ± 22.0	573 ± 5.5	1 ± 0.0
DMTase	2 ± 0.5	319 ± 3.6	21631 ±13.5	2894 ± 9.6	1 ± 0.0

Shown above are the relative levels of viral gene expression as determined by qRT-PCR using the primers listed in Supplementary Table 1. The values were calculated by the ΔΔcT method (Livak and Schmittgen, 2001) using 18S rRNA as the reference. cDNA was prepared from RNA extracted from wt-infected cells at 2, 4, and 9 hr p.i., ts5-infected cells incubated at 30°C for 9 hr, and CHX-treated, wt-infected cells at 9 hr. qRT-PCR reactions were performed in triplicate using total RNA isolated from three independent infections. The values shown are the means ± standard deviations for a representative experiment.