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. Author manuscript; available in PMC: 2009 Aug 26.
Published in final edited form as: Lab Invest. 2007 Jul 16;87(9):938–950. doi: 10.1038/labinvest.3700658

Figure 1.

Figure 1

Human osteosarcoma expresses high levels of α-CaMKII. MG-63,143B and hMSC cells were used. Representative images from three experiments of (a) RT-PCR using CaMKII isoforms or actin-specific primers. (b) Western blots using antibodies directed against phosphorylated and total α-CaMKII and actin (c) Immunohistochemistry staining using a specific antibody directed against p-α-CaMKII. (d) Primary human osteosarcoma tissue was surgically removed, formalin-fixed and paraffin-embedded. H&E staining (left) shows osteosarcoma. Immunohistochemical staining (right) was performed using an antibody against p-α-CaMKII (brown), counterstained with hematoxylin (blue). NC, without immunoreactivity, is shown in the low left inset. Photomicrographs were obtained at × 400 magnification, and are representative of the four different patients.