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Flexible registration flowchart. (A) Atomic structure (tube representation) and corresponding feature vectors (red spheres). (B) Cryo-EM volumetric map (isosurface) and corresponding reduced representation (green spheres). (C) Rigid body registration of the multi-resolution datasets is achieved through the alignment of the feature vectors; arrow indicates divergent conformational states in the data sets. (D) Flexible fitting of the atomic structure into the cryo-EM map.
