Figure 5.

Islet morphology and gene expression in GPR39-deficient mice. A, Immunohistochemistry of representative islets from WT mice (upper panels) and Gpr39(−/−) mice (lower panels). Indirect immunofluorescence for insulin (i and v), glucagons (ii and vi), somatostatin (iii and vii), and PP (iv and viii) was performed on fixed pancreas tissue. B, The islet cell area as determined in hematoxylin and eosin-stained pancreatic slides was 1.0 ± 0.2% in Gpr39(+/+) and 0.94 ± 0.2% in Gpr39(−/−). C, Quantification of the β-cell number per islets performed by histomorphometric analysis.