Figure 6.

Role of CRE2, CRE3, and C/EBPβ sites in predifferentiated granulosa cells. CAT constructs controlled by WT −96Star promoter or its mutations shown in Fig. 4, were examined in E₂-primed granulosa cells, with or without 6 h 100 ng/ml FSH treatment. The activity values (mean ± sd) are presented relative to the activity of −96Star in FSH treated cells. Activity levels were statistically significant when compared with the respective values obtained for the −96Star construct: a, P < 0.001; b, P < 0.05; c, P > 0.1.