Figure 3.
b5 expression in adrenal microsomes of neonatal rhesus macaques from birth to 3 months of age. A, Densitometric analysis of Western immunoblots of b5 (pmol/μg) expression in perinatal and neonatal rhesus adrenal microsomes. Band intensities were estimated by densitometry, and concentrations were based on recombinant rat b5 standard curves (0.01–1 pmol in each gel). Levels of b5 increased with age (R2 = 0.63; P < 0.01). B, Representative Western immunoblot of b5 expression in rhesus neonatal adrenal microsomes (20 μg/lane) used for densitometry to detect age-related changes.